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Conditions for isolation of high quality total RNA from European linden  (Tilia cordata) leaves and pollen were determined. Pure total RNA was  isolated from linden leaves utilizing Qiagen plant mini kit while total RNA  isolated from linden pollen using this method was degraded. Successful  isolation of total RNA from both linden pollen and leaves, however, was  achieved following TRIZOL™ preparation of total RNA. Total RNA isolated using  TRIZOL™ was contaminated with genomic DNA but treatment with enzyme DNase, in  solution or on-column, efficiently removed the genomic DNA. Furthermore,  conditions for elimination of genomic DNA contamination on-column and  isolation of pure total RNA from leaves were optimized. Isolated total RNA  from both leaves and pollen was used successfully in firstand second-strand  cDNA synthesis reactions, as well as, in RT-PCR, demonstrating that the total  RNA isolated using this method is functional. In conclusion, pure and  functional total RNA from Tilia cordata leaves and pollen (27.8 ± 7.9μg/g  leaves; 25.7 ± 1.1μg/g pollen) can be obtained and applicable for further  molecular biology studies

Isolation of functional total RNA from Tilia cordata leaves and pollen