Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells | Zendy

Mira Milisavljević | Zendy; Gordana Timotijević | Zendy; Dragaikolić | Zendy; Jelena Samardžić | Zendy; Vesna Maksimović | Zendy

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Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells

Author(s) -

Mira Milisavljević,

Gordana Timotijević,

Dragaikolić,

Jelena Samardžić,

Vesna Maksimović

Publication year - 2011

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc110120106m

Subject(s) - pepstatin , immunocytochemistry , extracellular , western blot , recombinant dna , biochemistry , chemistry , cell culture , enzyme , nicotiana tabacum , microbiology and biotechnology , cell , cell wall , biology , gene , protease , genetics , endocrinology

The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extra- cellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained re- sults, potential functions of FeAPL1 are discussed.

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