The role of ATP in the function of human ORC4 protein | Zendy

Aleksandra Divac Rankov | Zendy; Branko Tomić | Zendy; Jelena KušićTišma | Zendy

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The role of ATP in the function of human ORC4 protein

Author(s) -

Aleksandra Divac Rankov,

Branko Tomić,

Jelena KušićTišma

Publication year - 2010

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc090724019d

Subject(s) - atp hydrolysis , cofactor , biochemistry , dna , function (biology) , chemistry , oligonucleotide , protein subunit , atpase , mutant , microbiology and biotechnology , enzyme , biology , gene

Hu man origin recog nition co mplex 4 (ORC4) pro tein, a subunit of the origin recognition complex, belongs to the AAA+ superfamily of adenosine triphosphate (ATP) ases. Proteins belongi ng to this fam ily require ATP for their function and intera ctions with ATP le ad to c onformational ch anges in them or i n th eir partners. Human O RC4 pro tein indu ces structural c hanges in DNA substrates, promoting renaturation and fo rmation of non- canonical struc- tures, as w ell a s conver sion of single-stra nded into multi-stranded oligo nuc- leotide struct ures. The ai m of this study wa s to further investigate the role of ATP in the function of hu man ORC4 protein. For this purpose, a mutant in the conserved Walker B motif of ORC4, which is able to bind but not to hydrolyze ATP, was con structed and it s activity in D NA restruc turing reactions w as investigated. The obtained results showed that ATP hydrolysis is not necessary for the function of hu man ORC4. It is proposed that ATP ha s a struct ural role as a cofactor in the function of human ORC4 as a DNA restructuring agent.

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