EPR study of the production of oh radicals in aqueous solutions of uranium irradiated by ultraviolet light

The aim of the study was to establish whether hydroxyl radicals ( • OH) were produced in UV-irradiated aqueous solutions of uranyl salts. The production of 'OH was studied in uranyl acetate and nitrate solutions by an EPR spin trap method over a wide pH range, with variation of the uranium concentrations. The production of • OH in uranyl solutions irradiated with UV was unequivocally demonstrated for the first time using the EPR spin-trapping method. The production of • OH can be connected to speciation of uranium species in aqueous solutions, showing a complex dependence on the solution pH. When compared with the results of radiative de-excitation of excited uranyl ( * UO 2+ 2 ) by the quenching of its fluorescence, the present results indicate that the generation of hydroxyl radicals plays a major role in the fluorescence decay of * UO 2+ 2 . The role of the presence of carbonates and counter ions pertinent to environmental conditions in biological systems on the production of hydroxyl radicals was also assessed in an attempt to reveal the mechanism of * UO 2+ 2 de-excitation. Various mechanisms, including • OH production, are inferred but the main point is that the generation of • OH in uranium containing solutions must be considered when assessing uranium toxicity.