Isolation of thymus gland fractions and the determination of their biological activity | Zendy

Milena Radeta | Zendy; Jasovakovic | Zendy; Milanka Vico-Stevanovic | Zendy; Slobodanka Simic | Zendy; Aleksandar Pirozkov | Zendy

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Isolation of thymus gland fractions and the determination of their biological activity

Author(s) -

Milena Radeta,

Jasovakovic,

Milanka Vico-Stevanovic,

Slobodanka Simic,

Aleksandar Pirozkov

Publication year - 2007

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc0703225r

Subject(s) - glycolipid , chemistry , phospholipid , biuret test , peptide , fraction (chemistry) , chromatography , biochemistry , in vivo , in vitro , biology , urea , microbiology and biotechnology , membrane

A calf thymus extract was prepared and fractionated into lipid and non-lipid fractions. The non-lipid fraction was isolated from the calf thymus extract using the Folch method. The components isolated from the non-lipid fraction were characterized by IR, NMR, biuret and HPLC method. The results of the analyses in- dicated the presence of peptides. The lipid fraction contained phospholipids, glyco- lipids and neutral lipids. The biological activity of both the isolated lipid and peptide fractions was determined by the in vivo hemolytic plaques method in Wistar rats with an involuted thymus. The peptide and phospholipid fractions of the thymus ex- tract showed a significant increase of hemolytic plaques. The glycolipid and neutral lipid fraction failed to express a significant immunological response.

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