High-performance liquid chromatographic separation of surface flavonoid aglycones in Artemistia annua L. and Artemisia vulgaris L | Zendy

Mileikolova | Zendy; Reneta Gevrenova | Zendy; Stephanka Ivancheva | Zendy

Open Access

High-performance liquid chromatographic separation of surface flavonoid aglycones in Artemistia annua L. and Artemisia vulgaris L

Author(s) -

Mileikolova,

Reneta Gevrenova,

Stephanka Ivancheva

Publication year - 2004

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc0407571n

Subject(s) - artemisia annua , chemistry , chromatography , flavonoid , quercetin , high performance liquid chromatography , ether , artemisia , methanol , botany , organic chemistry , biology , antioxidant , artemisinin , plasmodium falciparum , malaria , immunology

External flavonoid aglycones from Artemisia annua L. and Artemisia vulgaris L. were analyzed by the HPLC method. The mobile phase was composed of t-butanol-methanol-20 mmol l - 1 phosphate buffer, pH 3.22. The linear gradient elution method within 22 min was applied. The main aglycones in A. annua and A. vulgaris are methyl ethers of quercetagetin and quercetin. Quercetagetin 3,6,7-trimethyl ether in A. annua and quercetin 3,7,3'-trimethyl ether in A. vulgaris are the most abundant compounds.

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