A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure | Zendy

Dragan B. Zlatković | Zendy; Dragica Jakovljević | Zendy; Djordje B. Zeković | Zendy; Miroslav Vrvić | Zendy

Open Access

A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure

Author(s) -

Dragan B. Zlatković,

Dragica Jakovljević,

Djordje B. Zeković,

Miroslav Vrvić

Publication year - 2003

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc0311805z

Subject(s) - saccharomyces cerevisiae , glycosidic bond , yeast , periodate , chemistry , polysaccharide , glucan , hydrolysis , enzymatic hydrolysis , mass spectrometry , nuclear magnetic resonance spectroscopy , enzyme , chemical structure , biochemistry , stereochemistry , organic chemistry , chromatography

The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry baker's yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1 3)-linked -D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1 3)--oligoglucosides, linked to the main chaing through (1 6)-glucosidic linkages. The low optical rotation as well as the 13 C-NMR and FTIR spectra suggest that the glycosidic linkages are in the -D-configuration.

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