Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent | Zendy

Dhruv K. Singh | Zendy; Bhavana Srivastava | Zendy; Archana Sahu | Zendy

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Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent

Author(s) -

Dhruv K. Singh,

Bhavana Srivastava,

Archana Sahu

Publication year - 2003

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc0309685s

Subject(s) - brucine , ajmaline , chemistry , reagent , chromogenic , molar absorptivity , tungstate , molybdate , absorbance , chromatography , spectrophotometry , nuclear chemistry , organic chemistry , biochemistry , medicine , physics , strychnine , optics , cardiology

A rapid and simple spectrophotometric procedure is described for the determina- tion of ajmaline and brucine. The method is based on the development of blue coloured product due to reduction of tungstate and/or molybdate in Folin Ciocalteu's reagent (FCR) by ajmaline and brucine in alkaline medium. The colour is stable for more than 48 h. The chromogenic reaction hasmax at 540 nm with molar absorptivity 1.64 10 4 and 2.37 10 3 l mol -1 cm -1 in the Beer's law range 1-8 gm l -1 and 10-100 gm l -1 for ajmaline and brucine, respectively.

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