Development of immunoradiometric assay for quantitative determination of free prostate-specific antigen | Zendy

Miroslava Janković | Zendy; Maja Kosanović | Zendy; Ljiljana Hajduković | Zendy; Snežana Golubović | Zendy

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Development of immunoradiometric assay for quantitative determination of free prostate-specific antigen

Author(s) -

Miroslava Janković,

Maja Kosanović,

Ljiljana Hajduković,

Snežana Golubović

Publication year - 2005

Publication title -

jugoslovenska medicinska biohemija

Language(s) - English

Resource type - Journals

eISSN - 1452-8193

pISSN - 0354-3447

DOI - 10.2298/jmh0502129j

Subject(s) - immunoradiometric assay , prostate specific antigen , chromatography , chemistry , prostate , antigen , detection limit , epitope , antibody , radioimmunoassay , microbiology and biotechnology , medicine , immunology , biology , biochemistry , cancer

Summary: In this study we reported the development and analytical validation of new assay for quantitative determination of free prostate-specific antigen, fPSA. It is formulated as one step, two-site »sandwich« immunoradiometric assay. Specificity of this assay was achieved by using epitope-1-reactive anti-fPSA antibody as tracer antibody. Assay was calibrated against first international standard 96/668, and its detection limit was determined as 0.08 mg/L. Intra- and inter-assay coefficients of variation were 3.42’7.53% and 7.04’ 8.33%, respectively. Measured concentrations of serially diluted serum samples were close to the calculated concentrations, indicating good linearity with recovery percentage ranging from 98.7’107.4%. Analytical performance characteristics of fPSA assay speaks in favour of its use as a reliable tool in laboratory diagnostics relating to prostate deseases.

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