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In a pot experiment, two fungal strains from soil, Aspergillus fumigatus and   Penicillium citrinum, were evaluated for their mycoherbicidal properties on   Amaranthus hybridus and Phyllanthus amarus using the biomass reduction   method. The experiment was set up in a completely randomised block design   made up of two weed species exposed to 20 treatments which consisted of the   concentrated crude extracts of Aspergillus fumigatus and Penicillium citrinum   at three levels as well as positive and negative controls, each in three   replications. The percentage biomass yields of Amaranthus hybridus and   Phyllanthus amarus were determined at 1, 3, 5 and 7 days after application   and biomass reductions were calculated. Data collected were subjected to   analysis of variance and significant means were separated using Duncan’s   multiple range test. Infections of the weeds occurred within 48 hours after   the application of the crude extract treatments. Significant differences (p ≤   0.001) were observed in the percentages of biomass yields of the two weed   species, especially at the highest inocula concentration (5% w/v) of the   crude extracts. The percentages of biomass yields of Amaranthus hybridus were   88.58% and 88.91%, while 69.79% and 81.34% were recorded for Phyllanthus   amarus after the application of the concentrated extracts of Aspergillus   fumigatus and Penicillium citrinum, respectively. The study shows that the   concentrated crude extracts of both Aspergillus fumigatus and Penicillium   citrinum had the potentials for use as biocontrol agents, with the fact that   extracts of Penicillium citrinum had the greatest impact on the biomass   yields of the two test weeds

Biocontrol potentials of crude extracts of soil fungi on Amaranthus hybridus and Phyllanthus amarus