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Effects of avocado seed extract (ASE) in different tris-extenders on sperm   and seminal oxidative stress indices of vitrified spermatozoa of West African   Dwarf (WAD) goats during vitrification were assessed. Semen samples were   diluted with varying levels (0, 5, 10, 15 and 20 mL/100 mL of diluent) of ASE   in tris citric acid (TCA), tris sodium phosphate (TSP) and tris sodium   citrate (TSC) extenders while a tris-extender without citric acid, sodium   phosphate and sodium citrate served as control. Diluted semen samples were   vitrified and preserved. The results revealed higher (P<0.05) progressive   sperm motility in TCA extenders at 10%, 15% and 20%, and TSP extender at 20%   ASE. Acrosome integrities were higher (P<0.05) in the different   tris-extenders at 15% and 20% ASE. TCA, TSP and TSC extenders had higher   (P<0.05) membrane integrities at 10%, 15% and 20% ASE. TSP extenders had   higher (P<0.05) live sperm at 20% ASE. TCA extenders had higher acrosome   reaction while TSP at 20% ASE and TSC at 15% and 20% of ASE had higher sperm   capacitation. TCA extender at 20% ASE had reduced malondialdehyde (MDA)   concentrations while higher acrosin activities were observed at 20% ASE in   TCA extender (P<0.05). The findings indicated that 20% ASE in the   tris-extenders improved sperm and oxidative stress indices and the   tris-extenders were ranked best in this order: TCA > TSP > TSC

Effects of avocado seed extract in different trisextenders on sperm and oxidative stress indices of vitrified goat spermatozoa