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Reactive oxygen species (ROS) produced during stress responses are implicated   in a number of cellular responses including morphogenesis. The present study   was undertaken to study the changes in antioxidative enzymes during in vitro   morphogenesis of groundnut from de-embryonated cotyledon explants cultured on   Murashige and Skoog’s medium supplemented with 5.0 mg l-1 benzyl-adenine and   2.0 mg l-1 2,4-dichlorophenoxyacetic acid. During the early in vitro   ontogenic stages of groundnut, the activity of peroxidase (POD) and   polyphenol oxidase (PPO) increased from stage 0 (0 day) to stage II (14 days)   and decreased during stage III (25 days) and stage IV (45 days). The activity   of superoxide dismutase (SOD) showed an inverse trend. The results could be   correlated with the acquisition of competence, de-differentiation, division   and induction which occurred during shoot organogenesis. Histological studies   also showed that the mode of in vitro morphogenesis from the groundnut   explants was via shoot organogenesis. In light of the above study, it could   be concluded that the change in activity of the antioxidative enzymes studied   could be used as a marker to characterize the mode of plant regeneration

In vitro regeneration of groundnut: Changes in antioxidative enzymes and histological studies