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The absorption and fluorescence emission spectra of cyanidin and cyanidin   3-O-β-glucopyranoside (Cy3Glc) at pH 5.5 in aqueous solution have been   studied. The most effective fluorescence excitations of cyanidin were at the   UV absorption maxima at 220 and at 230 nm and at higher wavelengths at 270   and at 280 nm. Cyanidin exhibits fluorescence emission maxima at 310 nm and   in the visible range at 615 nm. For the Cy3Glc most effective fluorescence   excitation was at 220 and at 230 nm and at higher wavelengths at 300 and at   310 nm. Cy3Glc has fluorescence emission spectra with the maximum at 380 nm   and does not show fluorescence emission in the visible range. If compare   fluorescence emission spectra of cyanidin and Cy3Glc it can be seen that   fluorescence emission intensity for cyanidin is significantly higher than   that for Cy3Glc. These results reveals the impact of 3-glucosidic   substitution at C-3 of aglycone (to form Cy3Glc) on the significantly   decrease in fluorescence emission intensity, and disappearance of the   fluorescence emission in visible wavelength range

Investigation of fluorescence properties of cyanidin and cyanidin 3-o-β-glucopyranoside