The identification and quantification of bioactive compounds from the aqueous extract of comfrey root by UHPLC-DAD-HESI-MS method and its microbial activity | Zendy

Vesna Savić | Zendy; Saša Savić | Zendy; Vesikolić | Zendy; Ljubiša Nikolić | Zendy; Stevo Najman | Zendy; Jelena Lazarević | Zendy; Aleksandra Djordjevic | Zendy

Open Access

The identification and quantification of bioactive compounds from the aqueous extract of comfrey root by UHPLC-DAD-HESI-MS method and its microbial activity

Author(s) -

Vesna Savić,

Saša Savić,

Vesikolić,

Ljubiša Nikolić,

Stevo Najman,

Jelena Lazarević,

Aleksandra Djordjevic

Publication year - 2014

Publication title -

hemijska industrija

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.147

H-Index - 19

eISSN - 2217-7426

pISSN - 0367-598X

DOI - 10.2298/hemind131202013s

Subject(s) - rosmarinic acid , ellagic acid , chemistry , antimicrobial , chromatography , allantoin , aqueous extract , traditional medicine , biochemistry , organic chemistry , polyphenol , antioxidant , medicine

In this study the qualitative, quantitative and microbial determination of the aqueous extract of comfrey root was done. The qualitative and quantitative analyses were done by the UHPLC-DAD-HESI-MS method. As major bioactive compounds, allantoin, rosmarinic acid and ellagic acid were identified and their quantification was also done. The obtained results showed a high content of allantoin, ellagic acid and rosmarinic acid (8.91, 7.4 and 12.8%, respectively) which indicated that the comfrey root can be used as a source for the isolation of these three compounds. The results obtained by the determination of the antimicrobial activity showed that Escherichia coli ATCC8739 and Salmonela typhimirium ATCC6538 were most sensitive to the aqueous extract of comfrey root. The results showed that allantoin did not express the antimicrobial activity on all the investigated bacteria species, and based on this it can be concluded that allantoin is not responsible for the antimicrobial activity of the aqueous extract of comfrey root. [Projekat Ministrstva nauke Republike Srbije, br TR-34012

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