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In this study the qualitative, quantitative and microbial determination of   the aqueous extract of comfrey root was done. The qualitative and   quantitative analyses were done by the UHPLC-DAD-HESI-MS method. As major   bioactive compounds, allantoin, rosmarinic acid and ellagic acid were   identified and their quantification was also done. The obtained results   showed a high content of allantoin, ellagic acid and rosmarinic acid (8.91,   7.4 and 12.8%, respectively) which indicated that the comfrey root can be   used as a source for the isolation of these three compounds. The results   obtained by the determination of the antimicrobial activity showed that   Escherichia coli ATCC8739 and Salmonela typhimirium ATCC6538 were most   sensitive to the aqueous extract of comfrey root. The results showed that   allantoin did not express the antimicrobial activity on all the investigated   bacteria species, and based on this it can be concluded that allantoin is not   responsible for the antimicrobial activity of the aqueous extract of comfrey   root. [Projekat Ministrstva nauke Republike Srbije, br TR-34012

hemijska industrija

The identification and quantification of bioactive compounds from the aqueous extract of comfrey root by UHPLC-DAD-HESI-MS method and its microbial activity