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The present study deals with the extraction of digoxin (Dgx) from chloroform   and trichloroethylene extracts of the secondary glycosides of fermented   foxglove (Digitalis lanata Ehrh.) foliage by liquid-liquid extraction. The   extraction degree (ED) of Dgx achieved by maceration and percolation using   10% vol. aqueous ethanol solutions were higher than 95%. Using   trichlorethylene and chloroform, the ED of Dgx of about 100% and 96%,   respectively from the liquid ethanolic extracts (macerate or percolate) were   achieved by the four-cycle extraction. Fifteen separating funnels were   employed for the liquid-liquid extraction. Three different four-component   two-phase systems (ethanol:water - chloroform:ethyl acetate, ethanol:water -   chloroform:trichloroethylene and ethanol:water - trichloroethylene:ethyl   acetate) were tested as an extracting solvent to get the final product   having more than 98% of Dgx. The initial amount of the chloroform or   trichloroethylene extract in the light phase was varied between 5 and 25   g/L, while the volume ratio of light and heavy phases was in the range of   1:1 to 1:2. The best Dgx yield of 98% was achieved with the system   ethanol:water - chloroform:trichloroethylene 35:15:20:30 at the volume ratio   of the phases of 1:1.1 and at the initial amount of the extract of 15 g/L.   Purity of the separated digoxin was 99.8 %. [Projekat Ministarstva nauke   Republike Srbije, br. TR-34012

Separation of digoxin by luiquid-luiquid extraction from extracts of foxglove secondary glycosides