Open AccessInhibitory effect of retinol acetate on horseradish peroxidase
Author(s) -
R Vladan Djuric,
R Nebojša Deletić,
Vesnа Stаnkov-Jovаnović,
Miljan Simonović
Publication year - 2013
Publication title -
hemijska industrija
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.147
H-Index - 19
eISSN - 2217-7426
pISSN - 0367-598X
DOI - 10.2298/hemind120602095d
Subject(s) - chemistry , horseradish peroxidase , peroxidase , hydrogen peroxide , radical , lactoperoxidase , enzyme , cofactor , catalysis , antioxidant , vitamin , benzidine , peroxide , ascorbic acid , biochemistry , photochemistry , organic chemistry , food science
Primary role of peroxidase enzyme is to decompose endogenous hydrogen peroxide, when oxygen radical is being replaced by a less potent radical, which is its cosubstrates oxidized form. During this study, catalytic activity of horseradish peroxidase has been observed in the presence of antioxidants from vitamin group, such as C, E and A, i.e. their water-soluble forms. It was found that vitamin E showed no effect on the enzyme activity and fate of cosubstrate radicals from the group of benzidine derivatives. Vitamin C proceeds enzymatic reaction showing its antioxidative character, and absorbs electrons from radicals, bringing cosubstrate back to its relaxed state. On the other hand, vitamin A plays a role of uncompetitive peroxidase inhibitor, which is visible through decreasing initial rate of catalytic reaction, and is reflected as virtual decrease of enzyme concentration. Furthermore, it prolongs life of endogenous hydrogen peroxide, which could potentially lead to oxidative stress of cells. This inhibitory effect can be used in analytical purpose, for determination of retinol acetate content in a sample