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The silkworm, Bombyx mori L. is a poikilothermic organism whose growth and   development is adversely affected by high temperatures. Rearing of silkworm   at high temperature conditions of tropical countries like India causes   extensive crop loss by reducing cocoon yield. Breeding new silkworm breeds   tolerant to high temperatures is the most effective and economical method to   increase cocoon yield. Introduction of stable genotypes in such breeding   programs and knowledge on the relationships between cocoon yield and other   traits would be more advantageous in increasing cocoon yield at high   temperatures. Therefore, in this study twenty silkworm genotypes were reared   in five artificially simulated environments with different temperatures   ranging from 25°C to 38°C. Based on coefficient of variation (%) values, the   traits cocoon yield / 10,000 larvae by weight (CY) and cocoon weight showed   maximum and minimal variation among the traits in all the five environments.   Analysis of variance revealed a substantial genetic variability among the   genotypes for most of the traits studied. The results of combined analysis of   variance showed significant differences over traits indicating that the   traits are influenced by genotype × environment interactions. Stability   analysis with respect to CY showed higher mean value compared to grand mean,   regression coefficient equal to unity and less deviation from regression in   ATR16, CSR17, BHR2, S-5 and D13 genotypes, suggesting that they are   consistent with the CY over the different environments. Clustering based on   the stability parameters resulted in separation of twenty silkworm genotypes   into 5 clusters based on their ability to tolerate high temperatures.   Correlation studies demonstrated positive and significant (P < 0.05)   correlations between CY and other traits. Pupation rate was most positively   associated with CY followed by larval weight, cocoon weight, cocoon shell   weight and cocoon shell percent. Path coefficient analysis also revealed that   the pupation rate had the maximum direct effect on CY followed by cocoon   shell weight and larval weight. Cocoon shell percent and cocoon weight   exhibited negative direct path effect on cocoon yield / 10,000 larvae by   weight. Therefore, the selection based on pupation rate, larval weight and   cocoon shell weight can be effective in improving cocoon yield in silkworm at   different environments with varying temperature

Stability and path analysis for yield and related traits in silkworm, (Bombyx mori L.) reared under stress conditions