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Plant defensins are promising future strategy as antimicrobial agents;   however the number of characterized plant defensins is very low. We isolated   and studied the expression of plant defensins in six plants from Taif   region. Using RT-PCR and two pairs of common as well as three pairs of   specific primers, the defensin gene expression was analyzed. Plants showed   differences in defensin gene expression in floral buds and leaves, though   floral buds represented higher gene expression. The amino acid sequence of   the six isolated cDNA sequences showed high similarity with other defensin   accessions from the nucleotide database, especially in the cysteine rich   motif. Phylogenetic analysis revealed that the isolated sequences share the   common features of plant defensins, especially the 8 conserved cysteines.   The results of this study help to detect some valuable Saudi plant sources   for the development of natural peptides as a replacement for chemical   antibiotics

Defens in gene expression in some plant sources of Taif