Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker | Zendy

Lijing Chen | Zendy; Hongmei LiByarlay | Zendy; Shaokun Sun | Zendy; Muhammad Irfan | Zendy; Jingwei Lin | Zendy; Ming Zhong | Zendy; Hui Ma | Zendy; Zhi-Fu Guo | Zendy; Tianlai Li | Zendy

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Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker

Author(s) -

Lijing Chen,

Hongmei LiByarlay,

Shaokun Sun,

Muhammad Irfan,

Jingwei Lin,

Ming Zhong,

Hui Ma,

Zhi-Fu Guo,

Tianlai Li

Publication year - 2015

Publication title -

genetika

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.24

H-Index - 15

eISSN - 1820-6069

pISSN - 0534-0012

DOI - 10.2298/gensr1502425c

Subject(s) - biology , quantitative trait locus , genetic linkage , genetic marker , genetics , gene mapping , locus (genetics) , population , molecular marker , genetic distance , restriction fragment length polymorphism , ecori , inbred strain , restriction enzyme , genetic variation , genotype , chromosome , gene , sociology , demography

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium

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