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A genetic linkage map of lily was constructed using RILs (recombinant inbred   lines) population of 180 individuals. This mapping population was developed   by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars   through single-seed descent (SSD). SRAPs were generated by using restriction   enzymes EcoRI in combination with either MseI. The resulting products were   separated by electrophoresis on 6% denaturing polyacrylamide gel and   visualized by silver staining. The segregation of each marker and linkage   analysis was done using the program Mapmaker3.0. With 50 primer pairs, a   total of 189 parental polymorphic bands were detected and 78 were used for   mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups.   The number of markers in the linkage groups varied from 1 to 12. The length   of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval   distance range from 9.4 cM to 345.4 cM individually. The mean marker interval   distance between markers was 27.4 cM. The map developed in the present study   was the first sequence-related amplified polymorphism markers map of lily   constructed with recombinant inbred lines, it could be used for genetic   mapping and molecular marker assisted breeding and quantitative trait locus   mapping of Lilium

Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker