Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules | Zendy

M. Terzić | Zendy; Bratislav P. Marinković | Zendy; D. Šević | Zendy; J J Jureta | Zendy; Aleksandar R. Milosavljević | Zendy

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Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules

Author(s) -

M. Terzić,

Bratislav P. Marinković,

D. Šević,

J J Jureta,

Aleksandar R. Milosavljević

Publication year - 2008

Publication title -

facta universitatis - series physics chemistry and technology

Language(s) - English

Resource type - Journals

eISSN - 2406-0879

pISSN - 0354-4656

DOI - 10.2298/fupct0801105t

Subject(s) - biomolecule , laser induced fluorescence , fluorescence , laser , fluorescence spectroscopy , materials science , spectrograph , nanosecond , spectrometer , streak camera , optics , chemistry , nanotechnology , physics , astronomy , spectral line

Our developments of the time-resolved laser-induced fluorescence (TR-LIF) detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB) with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules

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