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The effect of different matrix pretreatment of winter savory(Satureja montana   L.) on the supercritical CO2(SC-CO2) extraction - yield, composition and   antimicrobial activity of extracts and essential oil (EO) was investigated.   Herb matrix was submitted to conventional mechanical grinding, physical   disruption by fast decompression of supercritical and subcritical CO2 and   physical disruption by mechanical compression. The analyses of the essential   oil obtained by SC-CO2 extraction and hydrodistillation were done by GC/FID   method. Major compounds in winter savory EO obtained by SC-CO2 extraction   and hydrodistillation were: thymol (30.4-35.4% and 35.3%), carvacrol   (11.5-14.1% and 14.1%), γ-terpinene (10.2-11.4% and 9.1%) and p-cymene   (8.3-10.1% and 8.6%), respectively. The gained results revealed that   physical disruption of essential oils glands by fast CO2 decompression in   supercritical region (FDS) achieved the highest essential oil yield as well   as highest content of thymol, carvacrol and thymoquinone. Antimicrobial   activity of obtained winter savory SC-CO2 extracts was the same (FDS) or   weaker compared to essential oil obtained by hydrodistillation

Effect of matrix pretreatment on the supercritical CO2 extraction of Satureja montana essential oil