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The separation ability and retention of normal and reversed phase HPLC with,  respectively, three non-polar and two polar mobile phases, have been studied  by measuring the retention constants of a series of newly synthesized  estrone derivatives. The separation ability and retention are discussed in  terms of the nature of the solute, eluent and stationary phase. Good  correlation was found between the retention constants log k0 of newly  synthesized estrone derivatives obtained on C-18 column and log P calculated  by different methods

Separation and lipophilicity of some new steroid derivatives in normal- and reversed-phase high performance liquid chromatography