Optimal growth of Saccharomyces cerevisiae (PTCC 24860) on pretreated molasses for ethanol production: Application of response surface methodology | Zendy

Hoda Shafaghat | Zendy; Ghasem Najafpour | Zendy; Pouya Sirous Rezaei | Zendy; Mazyar Sharifzadeh | Zendy

Open Access

Optimal growth of Saccharomyces cerevisiae (PTCC 24860) on pretreated molasses for ethanol production: Application of response surface methodology

Author(s) -

Hoda Shafaghat,

Ghasem Najafpour,

Pouya Sirous Rezaei,

Mazyar Sharifzadeh

Publication year - 2010

Publication title -

chemical industry and chemical engineering quarterly

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.189

H-Index - 26

eISSN - 2217-7434

pISSN - 1451-9372

DOI - 10.2298/ciceq100201029s

Subject(s) - response surface methodology , sugar , chemistry , incubation , ethanol fuel , food science , ethanol , saccharomyces cerevisiae , incubation period , yeast extract , central composite design , sugar beet , yeast , fermentation , chromatography , biochemistry , biology , horticulture

Saccharomyces cerevisiae (PTCC 24860) growth on pretreated sugar beet molasses was an optimized via statistical approach. In order to liberate all monomeric sugars, pretreated sugar beet molasses with dilute acid was obtained. The influence of process parameters such as sugar concentration, nitrogen source, pH and incubation time on the cell growth were investigated by a design expert software with the application of a central composite design (CCD) under response surface methodology (RSM). The optimal culture conditions were pH of 5.3, incubation time of 24 h and medium composition of 35 g reduced sugars, 1.5 g NH4Cl and 1 g yeast extract per liter of the media. At optimal cell growth conditions and incubation time of 12 h, the maximum ethanol production of 14.87 g/L was obtained.

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