Assignment for genes encoding the terminal complement components to porcine chromosome

One of the major goals of the porcine genome projects is building a physical map. To assign the porcine genes encoding the complement components C6, C7, C8 and C9 to porcine chromosomes, we used a porcine 7000Rad Radiation Hybrid panel (IMpRH) containing 118 clones provided by INRA-University of Minnesota. It resulted in assignment of the porcine C6, C7 and C9 genes to chromosome 16q1.4, the porcine C8A and C8B genes to chromosome 6q3.1-q3.5 as well as the porcine C8G gene lonely to chromosome 1q2.13. Key words : IMpRH mapping, C6, C7, C8A, C8B, C8G, C9, pig Introduction The assignment of genes using radiation hybrid (RH) panels is an efficient way to map genes and markers as well as to integrate the linkage and cytogenetic maps of a species Hawken et al., 1999; Yerle et al., 1998( . RH mapping enhances )linkage map reliability because of unambiguous determination of marker order and provides a powerful tool for fine mapping Yerle et al., 1998( . Moreover, radiation )hybrid allows gene assignments without the detection of genetic polymorphism as needed for linkage mapping.