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For association between BMP-15 (exon2) gene polymorphisms and litter size  trait in Anglo-Nubian goat, PCR-SSCP technique was developed. Twenty-five  female Anglo-Nubian goats reared under Egyptian conditions were selected  according to their litter size. DNA from blood samples of these animals was  extracted to amplify 140-bp of the BMP-15 gene affecting litter size  production trait in goats. Based on the breeding value, 25 animals were  selected from the highest to the lowest litter size productivity. PCR  amplification size of the BMP-15 gene (140-bp) was genotyped in all animals.  PCR-SSCP analysis of the BMP-15 gene (140-bp) showed three various genotypes  BB, BM and MM with frequencies 0.46, 0.43 and 0.11, respectively. The  frequencies of the B and M alleles were 0.68 and 0.32, respectively. The  results indicated that the BB genotype was higher in litter size productivity  than the other genotypes with significant differences. The result of this  study confirmed that BMP-15 gene may be a strong candidate gene for further  applications in marker-assisted selection (MAS) for litter size in goats.

Polymorphism in BPM-15 gene and its association with litter size in Anglo-Nubian goat