Bovine respiratory disease complex (BRDC): Viral and bacterial pathogens in Serbia | Zendy

Vladimir Kurčubić | Zendy; Radojica Djoković | Zendy; Dejan Vidanović | Zendy; Milanko Šekler | Zendy; Kazimir Matović | Zendy; Zoran Ilić | Zendy; J Stojkovic | Zendy

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Bovine respiratory disease complex (BRDC): Viral and bacterial pathogens in Serbia

Author(s) -

Vladimir Kurčubić,

Radojica Djoković,

Dejan Vidanović,

Milanko Šekler,

Kazimir Matović,

Zoran Ilić,

J Stojkovic

Publication year - 2013

Publication title -

biotechnology in animal husbandry

Language(s) - English

Resource type - Journals

eISSN - 2217-7140

pISSN - 1450-9156

DOI - 10.2298/bah1301037k

Subject(s) - pasteurella multocida , enrofloxacin , biology , microbiology and biotechnology , bovine respiratory disease , virology , virus , pathogen , antibiotics , pasteurella , bacteria , ciprofloxacin , genetics

Pathogens causing BRDC in Serbia were investigated. Two herds of beef cattle with bovine respiratory disease were included, with twenty diseased calves (10 from each farm) were chosen for isolation of bacteria on artificial culture media and determination by aerobic cultivation. The most common bacterial pathogen was isolated was Pasteurella multocida. Diffusion method of sensitivity to antibiotics (antibiogram), revealed that Enrofloxacin and Floron were most efficient antibiotics against Pasteurella multocida isolates (100 % isolates sensitive on both antibiotics). From the all examined samples (n=20) using the method of Real Time PCR (RT-PCR and PCR) we determined the genome sequences of bovine respiratory syncytial virus (BRSV), but in none of the samples genome of bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BoHV-1). [Projekat Ministarstva nauke Republike Srbije, br. TR 31001]

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