Porcine stress syndrome (PSS) in mangalitsa pigs | Zendy

N. Stanišić | Zendy; S. Aleksić | Zendy; Di Liu | Zendy; Zoran Stanimirović | Zendy; Zhenhua Guo | Zendy; M. Petrović | Zendy; N. Delić | Zendy; Čedomir Radović | Zendy; Nenad Parunović | Zendy; Marija Gogić | Zendy

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Porcine stress syndrome (PSS) in mangalitsa pigs

Author(s) -

N. Stanišić,

S. Aleksić,

Di Liu,

Zoran Stanimirović,

Zhenhua Guo,

M. Petrović,

N. Delić,

Čedomir Radović,

Nenad Parunović,

Marija Gogić

Publication year - 2012

Publication title -

biotechnology in animal husbandry

Language(s) - English

Resource type - Journals

eISSN - 2217-7140

pISSN - 1450-9156

DOI - 10.2298/bah1204873s

Subject(s) - genotype , ryr1 , restriction fragment length polymorphism , biology , restriction enzyme , polymerase chain reaction , gene , endonuclease , microbiology and biotechnology , genetics , receptor , ryanodine receptor

Porcine stress syndrome (PSS) is one kind of molecular genetics defect which will cause malignant hyperthermia syndrome in pigs. It was reported that mutation of pig rynodine receptor (RYR1) gene is the main reason for PSS. The aim of this study was to test the RYR1 genotype of 10 Mangalitsa pigs using a polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) technique, which is a reliable and simple method for RYR1 gene testing. Extraction of DNA was done by using hair follicles. The results showed that the RYR1 genotype of all the 10 porcine cases were negative. These results suggested that Mangalitsa pig could be one of the porcine breeds selectively bred for medical and clinically experiments.

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