A study of biochemical polymorphism in carp (Cyprinus Carpio): Detect new alleles in transferrin

This study was carried out at the college of agriculture and marine science centre, Basrah university. The present study was conducted to investigate the existence of polymorphism at transferrin (Tf) locus in the Carp (Cyprinus carpio). A polyacrylamide gel electrophoresis (PAGE) under alkaline condition method was used to distinguish Carp Tf alleles. Use the gel documentation program in this study. Analysis of 116 animals revealed that all animals were polymorphic, showing many genotypes. There was very clear biodiversity in the Tf gene. Seven Tf genotypes consisting of 4 homozygote types (CC, DD, FF and GG) and two heterozygote types (CD, DG and FG) were detected. These fractions are controlled by co-dominant autosomal genes according to the Mendelian laws of inheritance. The highest gene frequencies were calculated 0.50 for Tf D, 0.26 for Tf F and 0.12 for C and G. thus, carp (Cyprinus carpio) assemblages consistently tended to be more predominant to D allele. Differences between expected number and observed number for transferrin genotypes were no significant. This is useful in genetic improvement process through the selection. As far as we know, this is the first large-scale analysis on the genetic polymorphism in carp (Cyprinus carpio). Polyacrylamide electrophoresis, the technique employed in this study, allows rapid and efficient screening for the presence of polymorphism in Tf.