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The goal of this work was to induce clinical sepsis in rats in order to  measure the following biochemical parameters (glucoses, triglycerides,  cholesterol, total proteins, albumins and creatinine). The experiments were  done on 104 male Wistar rats, body weight 190 to 240 g. The rats were divided  in four groups. Treated groups consisted of 28 animals while in the control  groups there were 20 animals. Animals were observed and sacrificed at 12, 24,  72 and 120 hours after surgery. In this model of sepsis (cecal ligation and  puncture - CLP) with pure and mixed bacterial cultures, significant changes  were noticed in all biochemical parameters. Significant hypoglycemia,  hypercholesterolemia, decreased concentration of urea and increased  concentration of creatinine were found in the first half of the experiment in  all groups of rats with sepsis (at 12 and 24 hours). Hyperproteinemia and  hypotriglyceridemia reached statistically significant values at 24 h in the  groups of rats in which sepsis was induced with pure bacteria culture. In the  other half of the experiment significant hypoalbuminemia was found in all  rats with sepsis (at 71 and 120 h). [Projekat Ministarstva nauke Republike  Srbije, br. TR 31071

Biochemical parameters in rats with an applied model of sepsis (cecal ligation and puncture) with pure and mixed bacterial culture