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Few studies were conducted to investigate the pathogenesis and to  characterize the histopathological alterations in the intestinal mucosa of  kids with cryptosporidiosis. In our study, 58 kids were presented for  necropsy, and the faeces of another 83 goat kids were studied. Animals were  aged between 1 day and 3 weeks. Infection was confirmed by oocyst  identification in the faeces (Ziehl-Neelsen stain modified by Henriksen).  Gross examination was followed by examination of the tissue using  histopathological procedures (Hematoxylin-Eosin stain), scanning electron  microscopy (SEM) and immunofluorescence. Pathological changes occurred mainly  in the ileum, but also jejunum and caecum of the infected animals, at the  apical surface of intestinal epithelial cells. Oocysts appeared as round or  oval formations, with a diameter ≤4.5 μm, sometimes covering the atrophic and  denudated free border of intestinal villi. Most histological and  morphological characteristics of infection with C. parvum are common on the  whole to other Cryptosporidium spp. Massive dentification of cryptospodiosis  in kids indicates the possible role of these animals as disease reservoirs  for other animals and for humans. This is the first study to characterize the  Cryptosporidium infection by confocal technique and SEM in Romania

Morphological, histopathological and immunofluorescence characterization of Cryptosporidium parvum natural infection in goat kids from Romania