Open AccessEndothelium-dependent relaxation of internal mammary artery produced by resveratrol
Author(s) -
Aleksandra Novakovic,
Bukarica-Gojkovic Ljiljana,
Miodrag Perić,
Dusan Nezic,
Bosko Djukanovic,
Aleksandar Lešić,
Marko Bumbaširević,
Markovic-Lipkovski Jasmina
Publication year - 2006
Publication title -
acta veterinaria
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 17
eISSN - 1820-7448
pISSN - 0567-8315
DOI - 10.2298/avb0603203n
Subject(s) - resveratrol , chemistry , glibenclamide , endothelium , pharmacology , vasodilation , 4 aminopyridine , charybdotoxin , biochemistry , potassium channel , biophysics , medicine , endocrinology , membrane potential , biology , diabetes mellitus
Resveratrol, a polyphenol present in wine, has been thought to be responsible for cardiovascular benefits associated with moderate wine consumption. It is also present in the plant Polygonum Cuspidatum. The mechanism of cardiovascular benefits probably includes vasorelaxation, antioxidant and anti-platelet effects of resveratrol. The mechanisms by which resveratrol causes vasodilatation are uncertain. The aim of this study was to investigate the mechanism(s) of resveratrol induced vasorelaxation in human internal mammary artery (HIMA) with endothelium. HIMA rings were precontracted by phenylephrine. Resveratrol induced relaxation of the HIMA rings with endothelium. LNAME, an inhibitor of NO synthase, and methylene blue, an inhibitor of guanylate cyclase, abolished relaxation of HIMA induced by resveratrol. Highly selective blocker of ATP-sensitive K channels, glibenclamide as well as a nonselective blocker of big Ca-sensitive K+ channels, charybdotoxin did not block resveratrol-induced relaxation of HIMA. 4-Aminopyridine and margatoxin, blockers of voltage-gated K+ (KV) channels, abolished endothelium-dependent relaxation of HIMA, induced by resveratrol. In conclusion, we have shown that resveratrol induces relaxation of HIMA with endothelium. It seems that NO and smooth muscle KV channels are included in this relaxation