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Ten phenolic acids, contained in the seeds of black cumin (Nigella sativa   L.), flax (Linum usitatissimum L.), pomegranate (Punica granatum L.) and   pumpkin (Cucurbita pepo L.) and their oil industry by-products, separated   into the free, esterified, and insoluble-bound forms, were quantitatively   analysed by reverse phase high performance liquid chromatography with   photodiode array detector. The chromatographic data were interpreted using   Principal Component Analysis (PCA). The PCA model with three principal   components (PC1-PC2-PC3) fitted well with 12 examined plant samples, allowing   their division into groups according to their origin. The total phenolic   variables could be represented by two PCs and for the pattern recognition of   the analysed samples, 13 phenolic variables are sufficient, including: free,   esterified and insoluble-bound forms of gallic and syringic acids, free   vanillic, insoluble bound p-coumaric, esterified p-hydroxybenzaldehide, and   free and insoluble-bound forms of p-hydroxybenzoic and trans-synapic acids.   This might have potential application in simplified screening of phenolic   compounds in seeds and their oil industry by-products or in food component   analysis or authenticity detection in such plant materials.[Projekat   Ministarstva nauke Republike Srbije, br. III 46010

Determination of phenolic acids in seeds of black cumin, flax, pomegranate and pumpkin and their by-products