Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems | Zendy

Mirjana Antov | Zendy; Branimir Jugović | Zendy; Milica M. Gvozdenović | Zendy; Zorica KneževićJugović | Zendy

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Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems

Author(s) -

Mirjana Antov,

Branimir Jugović,

Milica M. Gvozdenović,

Zorica KneževićJugović

Publication year - 2012

Publication title -

acta periodica technologica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.134

H-Index - 15

eISSN - 2406-095X

pISSN - 1450-7188

DOI - 10.2298/apt1243151a

Subject(s) - polyethylene glycol , dextran , yield (engineering) , extraction (chemistry) , chemistry , chromatography , phase (matter) , polyethylene , peg ratio , organic chemistry , materials science , finance , economics , metallurgy

This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase. [Projekat Ministarstva nauke Republike Srbije, br. 46010

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