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Antioxidant activity of mandarin (Citrus reticulata) peel
Author(s) -
Vesna Tumbas,
Gordana Ćetković,
Sonja Djilas,
Jasna ČanadanovićBrunet,
Jelena Vulić,
Željko Knez,
Mojca Škerget
Publication year - 2010
Publication title -
acta periodica technologica
Language(s) - English
Resource type - Journals
eISSN - 2406-095X
pISSN - 1450-7188
DOI - 10.2298/apt1041195t
Subject(s) - chemistry , dpph , hesperidin , radical , acetone , flavonoid , antioxidant , hydroxyl radical , food science , extraction (chemistry) , chromatography , organic chemistry , medicine , alternative medicine , pathology
Mandarin peel, a waste product coming from juice production, was extracted by conventional extraction with 70% acetone. Content of flavonoids in mandarin peel extract (MPE) was determined by HPLC. Hesperidin was the most dominant flavonoid. Free radical scavenging activity of MPE on stable DPPH radicals and reactive hydroxyl radicals was also evaluated. EC50 value determined in spectrophotometrical DPPH radical assay was 0.179 mg/ml, while this value in ESR spin trapping hydroxyl radical assay was 0.415 mg/ml. Also, MPE showed protective effects in stabilising sunflower oil during accelerated storage. The results indicated that mandarin peel can be a valuable source of natural antioxidants

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