Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate | Zendy

Draginja Pericin | Zendy; Senka Popović | Zendy; Zuzana Vastag | Zendy

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Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate

Author(s) -

Draginja Pericin,

Senka Popović,

Zuzana Vastag

Publication year - 2008

Publication title -

acta periodica technologica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.134

H-Index - 15

eISSN - 2406-095X

pISSN - 1450-7188

DOI - 10.2298/apt0839171p

Subject(s) - polyethylene glycol , penicillium roqueforti , protease , chemistry , factorial experiment , chromatography , partition coefficient , peg ratio , downstream processing , aqueous solution , fermentation , phosphate , aqueous two phase system , enzyme , penicillium , food science , biochemistry , organic chemistry , mathematics , statistics , finance , economics

The partitioning of acid protease produced by submerged fermentation of Penicillium roqueforti in the aqueous two-phase system polyethylene glycol/sodium phosphate was studied. In the system with 15% polyethylene glycol 4000/25% NaH2PO4 favourable partition coefficient and the top phase yield were obtained, 2.651 and 56.51%, respectively. The purification of acid protease from crude enzyme was optimized by optimizing separation factor. Completely randomized factorial design was applied and the system 15.54% PEG 4000/20% NaH2PO4 appeared to be optimal. Statistically calculated separation factor of 3.553, was similar to the experimentally obtained value (3.084), which indicates the possible application of the factorial design onto ATPS partition and purifycation

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