Bioconversion of apigenin-7-O-β-glucoside in aqueous two-phase system
Author(s) -
Sanja Ilić,
Sanja Djakovic,
Jelena Cvejic,
Mirjana Antov,
Zoran Zeković
Publication year - 2005
Publication title -
acta periodica technologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.134
H-Index - 15
eISSN - 2406-095X
pISSN - 1450-7188
DOI - 10.2298/apt0536197i
Subject(s) - apigenin , chemistry , glucoside , bioconversion , aqueous solution , chromatography , polyethylene glycol , peg ratio , aqueous two phase system , organic chemistry , flavonoid , medicine , fermentation , alternative medicine , finance , pathology , economics , antioxidant
The study is concerned with the conversion of apigenin-7-O-β-glucoside into apigenin in polyethylene glycol 6000 / dextran 20000 aqueous two-phase system by β-glucosidase. Apigenin was separated from apigenin-7-O-β-glucoside and β-glucosidase by their partition into opposite phases. In 14% PEG / 22.5% DEX aqueous two-phase system obtained yield of apigenin in top phase was 108%
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