Extraction of endo-pectinase activity from the culture filtrate of Polyporus squamosus by aqueous two-phase systems composed of low molecular mass polyethylene glycol and phosphate salt | Zendy

Draginja Peričin | Zendy; Ljiljana Radulović | Zendy; Senka Curcin | Zendy

Open Access

Extraction of endo-pectinase activity from the culture filtrate of Polyporus squamosus by aqueous two-phase systems composed of low molecular mass polyethylene glycol and phosphate salt

Author(s) -

Draginja Peričin,

Ljiljana Radulović,

Senka Curcin

Publication year - 2004

Publication title -

acta periodica technologica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.134

H-Index - 15

eISSN - 2406-095X

pISSN - 1450-7188

DOI - 10.2298/apt0435215p

Subject(s) - polyethylene glycol , pectinase , chemistry , downstream processing , phosphate , aqueous solution , extraction (chemistry) , chromatography , yield (engineering) , polyethylene , sodium , organic chemistry , enzyme , materials science , metallurgy

Separation of endo-pectinase activity from the culture filtrate of Polyporus squamosus - strain MMOL76, by aqueous two-phase partitioning technique in polyethylene glycol/sodium dihydrogen phosphate system was investigated as the first operation in the downstream processing of enzyme. The best results concerning the partitioning coefficient and the top phase yield were achieved in the polyethylene glycol 400/sodium dihydrogen phosphate system at the tie-line length 78.9 % at pH 3.8 (K = 8), with a 90% yield

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