Micronuclei and chromosomal aberrations in healthy tobacco chewers and controls: A study from Gujarat, India | Zendy

Beena P. Patel | Zendy; Pina J Trivedi | Zendy; Manisha M Brahmbhatt | Zendy; Shilin N. Shukla | Zendy; Pankaj Shah | Zendy; Sonal Bakshi | Zendy

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Micronuclei and chromosomal aberrations in healthy tobacco chewers and controls: A study from Gujarat, India

Author(s) -

Beena P. Patel,

Pina J Trivedi,

Manisha M Brahmbhatt,

Shilin N. Shukla,

Pankaj Shah,

Sonal Bakshi

Publication year - 2009

Publication title -

archive of oncology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.104

H-Index - 13

eISSN - 1450-9520

pISSN - 0354-7310

DOI - 10.2298/aoo0902007p

Subject(s) - micronucleus test , genotoxicity , chewing tobacco , buccal swab , medicine , micronucleus , smokeless tobacco , biomarker , tobacco smoke , dna damage , toxicology , physiology , pathology , cancer , environmental health , biology , tobacco use , genetics , toxicity , dna , population

Background: Tobacco chewing is attributed to oral cancer. Prediction of cancer development by genotoxicity analysis is a major challenge to identify tobacco users at greater risk. Therefore, present study aimed to analyze tobacco related genotoxic effects in chewers monitoring micronuclei (MN) and chromosome aberrations (CA). The biomarkers were compared with non chewer to (i) predict risk for genotoxicity, (ii) estimate synergistic effect of tobacco exposure with level of biomarkers, and (iii) identify best cellular site of measurements for genotoxicity assessment. Methods: Healthy tobacco chewers (n=47); and controls (n=48) were enrolled in the study. The peripheral blood lymphocyte and exfoliated buccal mucosa cells were studied for CA and micro nucleated cell count (MNC) respectively. An arbitrary unit was obtained for Lifetime Tobacco Exposure (LTE) using frequency/day multiplied by duration of years of tobacco use. Data were analyzed using SPSS statistical software. Results: MNC was significantly higher (p=0.001) in chewers than controls. CA was higher in chewers than controls. MNC can differentiate higher tobacco exposure in chewers than CA. Controls having MNC above cutoff level have greater risk of genotoxic exposition (95% C.I.; 1.462-23.26, p=0.012). Conclusion: The present study concludes that MNC is a better surrogate biomarker to predict genotoxicity than CA for tobacco exposure and DNA damage index in tobacco chewers

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