Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine | Zendy

Katica Bajin-Katic | Zendy; Karmen Stankov | Zendy; Zoran Kovačević | Zendy

Open Access

Changes of biochemical parameters in rat intestinal mucosa induced by methotrexate and effects of enteral administration of glutamine

Author(s) -

Katica Bajin-Katic,

Karmen Stankov,

Zoran Kovačević

Publication year - 2004

Publication title -

archive of oncology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.104

H-Index - 13

eISSN - 1450-9520

pISSN - 0354-7310

DOI - 10.2298/aoo0401035b

Subject(s) - methotrexate , intestinal mucosa , glutamine , crypt , enteral administration , alkaline phosphatase , pharmacology , intestinal epithelium , enzyme , acid phosphatase , glutaminase , chemistry , medicine , biochemistry , epithelium , pathology , parenteral nutrition , amino acid

BACKGROUND: Rapidly proliferating crypt cells of the intestinal epithelium, the precursors of the mature enterocytes, are extremely sensitive to the effects of cytostatic agents. We investigated the effects of the methotrexate on rat intestinal mucosa in order to get the information on biochemical indicators of intestinal damage. METHODS: Biochemical parameters were investigated in isolated intestinal mucosa of Sprague-Dawley rats, previously treated with methotrexate by intraperitoneal administration. Glutamine was dissolved in water and administered orally. RESULTS: The activity of glutaminase and alkaline phosphatase showed the enzymatic response to different doses of methotrexate. The activity of both enzymes was significantly lower in the mucosa of treated animals, compared to control group. CONCLUSION: Minimal mucosal damage and regeneration time is dose dependent and influenced by the dosage schedule of antitumor therapy

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