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Our objectives were to offer insight into the characteristics of the physical   environment in the River Danube in Serbia; and to show the relationship   between selected environmental factors and the composition and abundance of   macrophyte species. The macrophyte survey method followed the European   Standard EN 14184, applying Kohler’s five-level descriptive scale. Principal   component analysis was carried out to examine the variation in aquatic   vegetation, and to analyze the effect of environmental variables on the   aquatic vegetation, redundancy analysis was used. To build a simpler model   with fewer explanatory variables, yet sufficiently explaining species   variability, forward selection of environmental variables was done. Our   results confirmed that physical environmental factors significantly influence   the establishment and structure of macrophyte vegetation, with the level of   hydrological connectivity to the main river channel being the most important,   followed by the transparency of the water column, sediment texture and   water-flow velocity. In order to inspect the individual relation between   best-fitted plant species and each of selected environmental variables,   Spearman’s rank correlation coefficients were calculated. We have   distinguished plant species with preferences to specific combinations of   analyzed factors. Our results provide a background for future, more specific   studies on the macrophyte/habitat relationship. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III43002: Bio-sensing technologies and global systems   for the continual research and integrative ecosystem management

The relationship between habitat factors and aquatic macrophyte assemblages in the Danube river in Serbia