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Lycium ruthenicum Murr., which belongs to the family Solanaceae, is a resource   plant for Chinese traditional medicine and nutraceutical foods. In this   study, RNA sequencing was applied to obtain raw reads of L. ruthenicum fruit at   different stages of ripening, and a de novo assembly of its sequence was   performed. Approximately 52.45 million 100-bp paired-end raw reads were   generated from the samples by deep RNA-seq analysis. These short reads were   assembled to obtain 164814 contigs, and the contigs were assembled into   84968 non-redundant unigenes using the Trinity method. Assembled sequences   were annotated with gene descriptions, gene ontology, clusters of   orthologous group and KEGG (Kyoto Encyclopedia of Genes and Genomes)pathway   terms. Digital gene expression analysis was applied to compare   gene-expression patterns at different fruit developmental stages.   These results contribute to existing sequence resources for Lycium spp. during   the fruit-ripening stages, which is valuable for further functional studies   of genes involved in L. ruthenicum fruit nutraceutical quality

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De novo characterization of the Lycium ruthenicum transcriptome and analysis of its digital gene expression profiles during fruit development and ripening