Sex steroid application reverses changes in rat castration cells: Unbiased stereological analysis | Zendy

Nataša Nestorović | Zendy; Svetlana Trifunović | Zendy; Ivana Jarić | Zendy; Milica ManojlovićStojanoski | Zendy; Nataša Ristić | Zendy; Branko Filipović | Zendy; Branka ŠošićJurjević | Zendy; Verica Milošević | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Sex steroid application reverses changes in rat castration cells: Unbiased stereological analysis

Author(s) -

Nataša Nestorović,

Svetlana Trifunović,

Ivana Jarić,

Milica ManojlovićStojanoski,

Nataša Ristić,

Branko Filipović,

Branka ŠošićJurjević,

Verica Milošević

Publication year - 2016

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs151201070n

Subject(s) - endocrinology , testosterone propionate , medicine , stereology , testosterone (patch) , luteinizing hormone , hormone , intracellular , castration , follicle stimulating hormone , cytoplasm , biology , gonadotropin , chemistry , androgen , microbiology and biotechnology

The aim of the present study was to examine the morphometric characteristics of folliclestimulating hormone (FSH) and luteinizing hormone (LH) immunoreactive cells in the pituitaries of orchidectomized (Orx) and Orx testosterone- or estradiol-treated rats. Adult male Orx Wistar rats, 2 weeks post operation, received estradiol dipropionate (E) or testosterone propionate (T) for 3 weeks. Both controls, sham-operated (So) and Orx rats, were injected with solvent, in the same regime. Changes in the volume of pars distalis, the volumes of individual FSH- and LH-labeled cells, their numerical density and number were determined by unbiased design-based stereology. The FSH and LH intracellular content was estimated by relative intensity of fluorescence (RIF). We observed that Orx caused hyperstimulation of gonadotropic cells. Their volume, volume density, number, numerical density and intracellular hormone content significantly increased in comparison to So controls. Compared to Orx controls, T caused a significant decrease in the volume and volume density of gonadotropic cells and immunoreactive FSH and LH formations in their cytoplasm. The volume of the pars distalis, the numerical density and number of gonadotropic cells were not changed as compared to Orx controls. Estradiol treatment caused a significant increase in the volume of the pars distalis, decreases in cell volume, volume and numerical density of gonadotropic cells, and FSH and LH intracellular content in comparison to Orx controls. The number of FSH-labeled cells increased. In conclusion, both T and E reversed all of the examined parameters of gonadotropic cells of Orx rats to the level of So controls, except in number. [Projekat Ministarstva nauke Republike Srbije, br. 173009

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research