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The isolation of bacteria was carried out from samples of straw and chicken   manure, compost at various stages of the composting process and casing soil   used for growing button mushrooms. A preliminary screening of 108 bacterial   isolates for antagonistic activity against Trichoderma aggressivum f.   europaeum showed that 23 tested isolates inhibited mycelial growth of the   pathogenic fungus. Further screening with four indicator isolates of fungi   revealed that all 23 bacterial isolates inhibited the growth of T.   aggressivum f. europaeum, T. harzianum and T. koningii, while only 13   isolates inhibited the growth of T. atroviride. T. aggressivum f. europaeum   proved to be the most sensitive, with many bacterial isolates generating a   high percentage of growth inhibition. Only two bacterial isolates (B-129 and   B-268) were successful in inhibiting the growth of all 4 tested pathogens.   All 23 bacterial isolates were characterized as Gram-positive and   catalase-positive and were subjected to molecular identification based on the   partial sequence, the hypervariant region of the 16S rDNA. It was shown that   the obtained bacterial strains belong to Bacillus subtilis, B.   amyloliquefaciens, B. licheniformis and B. pumilus species. [Projekat   Ministarstva nauke Republike Srbije, br. 31043 i br. 173026

Isolation and identification of Bacillus spp. from compost material, compost and mushroom casing soil active against Trichoderma spp.