English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In order to address biosafety concerns regarding the constitutive expression   of foreign genes in crops, we applied a strategy aimed at confining foreign   gene expression in insect wounding sites of cotton. For this purpose, a plant   expression construct was designed by cloning the AoPR1 promoter   (pathogenesis-related protein gene isolated from Asparagus officinalis)   upstream from the insecticidal gene cry1Ac. The Turkish cotton cultivar cv.   STN-468 was transformed using the Agrobacterium tumefaciens strain LBA4404   containing the recombinant binary vector pRD400 harboring cry1Ac under a   wound-inducible promoter. The neomycin phosphotransferase (nptII) gene was   used as a selectable marker at a concentration of 100 mg/L. The primary   transformants were analyzed for T-DNA integration and expression using   standard molecular approaches. The efficacy of insecticidal gene control of   the AoPR1 promoter was investigated using leaf bioassays with 2nd instar   larvae of Helicoverpa armigera and Spodoptera littoralis. Positive primary   transformants from T0 progeny were further raised under greenhouse conditions   to obtain progeny (T1). The introduced gene was properly inherited and   expressed in T1 progeny. The mechanical wounding of plants resulted in   increased cry1Ac protein levels during 0-48 h of the wounding period. The   transgenic lines exhibited appreciable levels of resistance against targeted   insect pests in the leaf bioassays. The use of a wound-inducible promoter to   drive insecticidal gene expression is a valuable insect resistant management   strategy as gene expression will remain limited to the insect biting sites of   plant and crop, food and environmental concerns can be minimized

Development of insect-resistant cotton lines with targeted expression of insecticidal gene