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The hierarchical clustering method has been used for exploration of gene   expression and proteomic profiles; however, little research into its   application in the examination of expression of multiplecytokine/chemokine   responses to stimuli has been reported. Thus, little progress has been made   on how phytohemagglutinin(PHA) affects cytokine expression profiling on a   large scale in the human hematological system. To investigate the   characteristic expression pattern under PHA stimulation, Luminex, a multiplex   bead-based suspension array, was performed. The data set collected from human   peripheral blood mononuclear cells (PBMC) was analyzed using the hierarchical   clustering method. It was revealed that two specific chemokines (CCL3   andCCL4) underwent significantly greater quantitative changes during   induction of expression than other tested cytokines/chemokines after PHA   stimulation. This result indicates that hierarchical clustering is a useful   tool for detecting fine patterns during exploration of biological data, and   that it can play an important role in comparative studies

Evaluation by hierarchical clustering of multiple cytokine expression after phytohemagglutinin stimulation