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The aim of the study was to detect the activity and characterize potentially   fungistatic chitinases synthesized by rhizosphere bacteria identified as   Paenibacillus sp. M4. Maximum chitinolytic activity was achieved on the fifth   day of culturing bacteria in a growth medium with 1% colloidal chitin.   Analysis of a zymogram uncovered the presence of four activity bands in the   crude bacterial extract. The used three-stage protein purification procedure   resulted in a single band of chitinase activity on the zymogram. The purified   enzyme exhibited maximum activity at pH 6.5 and temperature 45oC, and thermal   stability at 40oC for 4 h. In terms of substrate specificity, it is an   exochitinase (chitobiose). The amino acid sequence obtained after mass   spectrometry showed similarity to chitinase A1 synthesized by Bacillus   circulans. The M4 isolate demonstrated the highest growth inhibiting activity   against plant pathogens belonging to the genera Fusarium, Rhizoctonia and   Alternaria. Fungistatic activity, although to a somewhat lesser degree, was   also demonstrated by purified chitinase. The obtained results confirm the   participation of the studied exochitinase in antagonism towards pathogenic   molds. However, the lower fungistatic effectiveness of the chitinases points   to the synergistic action of different metabolites in biocontrol by these   bacteria

The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4