Isolation of culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) and 16S rDNA diversity analysis
Author(s) -
Zongsheng Yuan,
Fang Liu,
Guofang Zhang
Publication year - 2015
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs141212063y
Subject(s) - biology , bacteria , bacillus (shape) , phyllostachys edulis , botany , 16s ribosomal rna , bamboo , isolation (microbiology) , rhizome , microbacterium , arthrobacter , enterobacter , pseudomonas , alcaligenes , microbiology and biotechnology , escherichia coli , biochemistry , genetics , gene
We analyzed culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) using traditional bacterial isolation and culture methods and then studied the colony characteristics and diversity with a 16S rDNA sequence analysis. We isolated 82 endophytic bacteria strains belonging to 47 species in 26 genera from the root, rhizome, stem and leaves of Moso bamboo species from populations on Wuyi Mountain, and in the Jiangle and Changting regions. There were significant differences in the composition of the culturable endophytic bacteria isolated from the different areas and from different tissues. The dominant bacteria strains from the Wuyi Mountain samples were Arthrobacter, Staphylococcus, Bacillus and Enterobacter, while the dominant bacteria from the Jiangle samples were Bacillus, Staphylococcus and Curtobacterium, and the dominant bacteria in the Changting samples were Alcaligenes, Pseudomonas, Staphylococcus and Bacillus. Our results demonstrate the abundant diversity of endophytic bacteria in Moso bamboo
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