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Stem cells from human exfoliated deciduous teeth (SHED) can be used as a   cell-based therapy in regenerative medicine and in immunomodulation. Pulp   from human deciduous teeth can be stored as a source of SHED. Glass ionomer   cements (GICs) are commonly used in restorative dentistry and in cavity   lining. GICs have lower biocompatibility and are cytotoxic for dental pulp   cells. In this study, seven commonly used GICs were tested for their   cytotoxic effects on SHED, for their potential to arrest mitosis in cells and   induce chromosome aberrations, and were compared with the effects of   composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had   significantly higher cytotoxic effects on SHED than composite. Only SHEDs   that have been treated with Fuji I, Fuji IX, Fuji plus and composite   recovered the potential for proliferation, but no chromosome aberrations were   found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were   in strong correlation with combined concentrations of released fluoride,   aluminum and strontium ions. Fuji I exhibited the lowest activity towards   SHEDs; it did not interrupt mitosis and did not induce chromosome   aberrations, and was accompanied by the lowest levels of released F, Al and   Sr ions. Projekat Ministarstva nauke Republike Srbije, br. ON175069, br.   ON175071 i br. ON175103

archives of biological sciences

Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations