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DNA damage detection in Ephestia kuehniella by RAPD-PCR Abstract - The random   amplified polymorphic DNA (RAPD) assay is a useful method for detecting   genotoxin-induced DNA damage. In the present study, this assay was evaluated   to measure the essential oil of Prangos ferulacea-induced DNA changes in   Ephestia kuehniella Zeller larvae. The third instar larvae of E. kuehniella   were exposed to 500 μl L-1 essential oil for 24 h. Forty random primers were   used for RAPD-PCR of which eleven produced unique polymorphic band patterns.   In comparison with control larvae, the essential oil-treated larvae showed   greater changes in RAPD profiles. This is the first report of an analysis of   the genotoxic effect of P. ferulacea essential oil against E. kuehniella   larvae using RAPD-PCR

archives of biological sciences

Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)