English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Tobacco is an important cash crop and an ideal experimental system for   studies of plant-pathogen interactions. Identification of tobacco resistance   (R) genes and resistance gene analogs (RGAs) is propitious to elucidate the   underlying resistant mechanisms. In recent years, the public tobacco EST   (expressed sequence tags) data set, which provides a rich source for   identifying expressed RGAs, has enlarged substantially. In this study, 149606   Uni-ESTs were assembled from 412325 tobacco ESTs available in GenBank,   scanned with 112 published plant R-genes protein sequences, and 1113   Nicotiana (tobacco) RGAs (NtRGAs) were identified. The majority of them   comprised the common R-genes domains, such as NBS-LRR, LRR-PK, LRR, PK and   Mlo, while we were unable to identify 109 RGAs using published domains of   R-genes. Upon sequence alignment, 1079 NtRGAs were allocated on 712 loci   within the Nicotiana benthamiana genome. A total of 78 simple sequence   repeats (SSRs) were identified from 72 NtRGAs, and out of 64 newly designed   primer pairs, 54 primer pairs generated clear bands upon PCR amplification   using tobacco genomic DNA. Only nine primer pairs displayed polymorphism in   24 varieties of tobacco, with 2-4 alleles per locus (2.56 alleles on   average), while 41 primer pairs were able to detect polymorphisms in six wild   species of genus Nicotiana, with 2-4 alleles per locus (2.61 alleles on   average)

Identification of expressed resistance gene analogs (RGA) and development of RGA-SSR markers in tobacco